Phenotype Detection and Quantification of Phenolic Compounds in Grapevine Leaves Using High-Performance Liquid Chromatography
Authors: J. Zhang, L. Wang, Y. Liu, H. Chen
Phenolic compounds play a crucial role in the defense mechanisms of grapevines against biotic and abiotic stresses. Accurate detection and quantification of these compounds are essential for understanding their physiological functions and for breeding programs. This study presents a robust high-performance liquid chromatography (HPLC) method for the simultaneous determination of major phenolic acids and flavonoids in grapevine leaves. The method was validated for linearity, precision, accuracy, and recovery. The optimized protocol was applied to analyze leaf samples from different grapevine varieties, revealing significant variations in phenolic profiles. The results demonstrate that the developed HPLC method is reliable and efficient for routine analysis of phenolic compounds in grapevine leaves, providing a valuable tool for phenotyping and quality assessment in viticulture.
