Phenotype Detection and Quantification of Phenolic Compounds in Grapevine Leaves Using High-Performance Liquid Chromatography

• Developed a validated HPLC method for simultaneous quantification of 12 phenolic compounds in grapevine leaves with high precision and recovery. • Significant genotypic differences in phenolic profiles were observed among grapevine varieties, indicating potential for marker-assisted selection. • The method is simple, cost-effective, and suitable for high-throughput phenotyping in grapevine breeding programs. • The optimized protocol can be adapted for other plant tissues and phenolic compounds, facilitating broader metabolomic studies.