• Radial glia are essential for vascular regeneration after spinal cord injury in zebrafish.
• Ablation of radial glia reduces vascular coverage and endothelial cell numbers at the injury site.
• Vegfaa signaling is downregulated after radial glia ablation, implicating it in the regenerative process.
• Notch and PI3K/Akt-mTOR pathways are potential downstream mediators of radial glia-driven vascular repair.