Small chemical molecule CPP promotes angiogenesis in surgically created severe lower limb ischemia and diabetes-induced limb vascular reduction models

• CPP, a small chemical molecule, directly promotes angiogenesis in vivo in severe lower limb ischemia and diabetes-induced limb vascular reduction models. • Subcutaneous multipoint injection of CPP significantly enhances blood perfusion and capillary density in ischemic limbs of mice. • The 1 mg/kg/day dose of CPP shows statistically significant improvement in capillary density compared to control, while the 10 mg/kg/day dose shows a trend but not significant. • CPP offers a promising therapeutic strategy for peripheral artery disease and chronic limb-threatening ischemia, potentially overcoming limitations of cell-based therapies.