• DRG neurons exhibit distinct response patterns to pain and itch stimuli, with multisensory neurons showing different response intensities compared to single-sensory neurons.
• Single-cell RNA sequencing reveals heterogeneity in DRG neuron subpopulations based on pain- and itch-related marker gene expression.
• Chronic pain and itch induce unique transcriptomic changes in distinct neuronal clusters, suggesting potential targets for therapeutic intervention.
• The study provides new insights into the molecular and cellular mechanisms underlying pain and itch, potentially informing novel treatment strategies.