Screening biomarkers for premature ovarian insufficiency based on cellular senescence and endoplasmic reticulum stress with experimental validation

• Identified 911 subtype-specific differentially expressed genes associated with cellular senescence and endoplasmic reticulum stress in premature ovarian insufficiency. • Aurora kinase A and actin binding protein were identified as potential key biomarkers with good diagnostic predictive performance. • M1 macrophages and resting dendritic cells showed significantly higher infiltration in premature ovarian insufficiency, suggesting immune involvement. • Animal experiments confirmed decreased expression of aurora kinase A and actin binding protein in ovarian tissue of premature ovarian insufficiency model mice.