Scaffold Compound T4015 Attenuates Pulmonary Fibrosis via Suppressing JAK/STAT and NF-κB Signaling

• T4015 is a novel dual inhibitor of JAK/STAT and NF-κB signaling pathways, effectively suppressing key phosphorylation events in vitro. • Transcriptomic analysis reveals broad downregulation of inflammation-related pathways, including TNF, IL-17, and Toll-like receptor signaling. • In a bleomycin-induced PF mouse model, T4015 improves survival, reduces collagen deposition, and lowers pro-inflammatory and profibrotic markers. • Molecular docking predicts strong binding of T4015 to multiple kinases in the JAK/STAT and NF-κB networks, supporting its dual-target mechanism.