Reviving hope: unlocking pancreatic islet immortality by optimizing a trehalose-based cryopreservation media and cell-penetrating peptide

• Trehalose combined with poly-L-proline (PLP) enables effective cryopreservation of pancreatic islets, maintaining high viability and functional integrity. • Cryopreserved islets exhibit glucose-responsive insulin secretion and reduced reactive oxygen species (ROS) levels, indicating preserved functionality. • Transplantation of thawed islets into diabetic mice restores euglycemia, evidenced by reduced fasting blood glucose and improved glucose tolerance. • This novel cryopreservation strategy addresses key limitations in islet transplantation, offering potential for improved Type 1 diabetes treatment.