Prevascularization of electrospun PCL/PLA scaffolds using human adipose-derived stem and endothelial cells enhances vascular integration and host angiogenesis in vivo

• Co-culture of AD-MSCs and HPAECs on electrospun PCL/PLA scaffolds supports formation of vessel-like structures in vitro, whereas monocultures do not. • Prevascularized scaffolds implanted on the CAM assay show functional anastomosis with host vasculature, as evidenced by perfused human microvessels containing chicken erythrocytes. • AD-MSC-seeded scaffolds significantly enhance host angiogenesis, with a 2.8-fold increase in vessel branching points, and co-culture scaffolds achieve a 3.5-fold increase compared to acellular controls. • The combination of stromal and endothelial cells is essential for prevascularization and functional vascular integration, highlighting the translational potential of this approach for tissue engineering.