Mechanomedicine-guided mechanical preconditioning of dental-derived stromal cells for tissue regeneration

• Mechanical preconditioning is a promising mechanomedicine-guided strategy to functionally prime dental-derived stromal cells (DSCs) for enhanced tissue regeneration. • DSCs respond to defined biophysical cues—including tensile/compressive forces, fluid shear stress, hydrostatic pressure, matrix stiffness, and topography—via mechanotransduction pathways that modulate lineage commitment and function. • Quantitative loading windows can be tailored to specific DSC subtypes and clinical indications, enabling more predictable therapeutic outcomes. • Translational success requires overcoming barriers such as stromal cell heterogeneity, donor variability, senescence, and the lack of standardized GMP-compatible manufacturing workflows.