Inhibition of USP22 by miR-200b-5p represses gastric cancer cell proliferation and migration by targeting the NF-κB signaling pathway

• miR-200b-5p directly targets USP22 by binding to its 3'UTR, leading to reduced USP22 expression in gastric cancer cells. • Overexpression of miR-200b-5p significantly inhibits gastric cancer cell proliferation and migration in vitro and suppresses tumor growth in vivo. • The tumor-suppressive effects of miR-200b-5p are mediated through downregulation of USP22, which in turn inhibits the NF-κB signaling pathway. • The inverse correlation between miR-200b-5p and USP22 expression in gastric cancer tissues suggests their potential as diagnostic biomarkers and therapeutic targets.