Inhibition and reversal of a TGF‑β1 induced myofibroblast phenotype by adipose tissue‑derived paracrine factors

• Whole adipose tissue conditioned media inhibits TGF-β1-induced fibroblast-to-myofibroblast differentiation, whereas ADSC or lipid conditioned media do not. • Hepatocyte growth factor (HGF) is present in adipose tissue conditioned media but is not responsible for the observed inhibition. • Adipose tissue conditioned media can reverse myofibroblast phenotype in TGF-β1-treated fibroblasts but not in scar-derived cell populations. • The study underscores the importance of whole adipose tissue in autologous fat grafting for hypertrophic scar treatment, warranting further mechanistic investigation.