Induction of apoptosis and autophagy in human glioblastoma cells by N-methylflindersine: insights into regulatory role of ERK pathway

• N-methylflindersine, an alkaloid from Micromelum falcatum, inhibits U87 glioblastoma cell growth by inducing apoptosis and autophagy. • The compound downregulates Bcl-2 and upregulates Bax, shifting the balance toward apoptosis. • It elevates the LC3-II/LC3-I ratio, indicating enhanced autophagic flux. • The antitumor effect is associated with downregulation of the ERK signaling pathway, offering a potential therapeutic target.