• IOSE80 cells exhibit stem cell characteristics including expression of stem cell markers and multilineage differentiation potential into adipocytes, osteoblasts, chondroblasts, and three germ layers.
• Transcriptome analysis reveals enrichment of stem cell proliferation and epithelial cell proliferation pathways, supporting the stemness of IOSE80 cells.
• IOSE80 cells can form oocyte-like cells in vitro and in vivo, suggesting potential for reproductive biology applications.
• In vivo transplantation of IOSE80 cells promotes ovarian repair, highlighting their therapeutic potential in regenerative medicine.