Hybrid endometrial-derived hydrogel and human endometrial organoids synergize for uterine regeneration in an immunocompetent murine model

• First in vivo evaluation of a hybrid hydrogel combining synthetic PuraMatrix® and endometrial extracellular matrix with human endometrial organoids for uterine regeneration. • The dual strategy significantly improved endometrial regeneration and fertility outcomes in an immunocompetent murine model of uterine damage. • Transcriptomic and histological analyses confirmed enhanced biocompatibility and tissue remodeling, supporting the translational potential of this approach. • This combined bioengineering strategy offers a promising new avenue for treating endometrial pathologies like Asherman syndrome and thin endometrium.