• hADSC-Exos effectively reduce UVB-induced skin photoaging by decreasing SA-β-gal-positive cells, p21 expression, ROS levels, and mtDNA common deletion in both HDFs and nude mice.
• The anti-photoaging mechanism involves activation of PINK1/Parkin-mediated mitophagy, as evidenced by increased PINK1, Parkin, and LC3bII/I ratio, and decreased p62.
• Silencing PINK1 via siRNA abolishes the protective effects of hADSC-Exos, confirming that PINK1/Parkin pathway is essential for their action.
• These findings support hADSC-Exos as a promising non-invasive therapeutic strategy for skin rejuvenation and photoaging treatment.