Official PDF Translation•Stem Cell Research & Therapy

Generation of biologically responsive colon-like intestinal tissue patches from human induced pluripotent stem cells using a rapid co-differentiation platform

Authors: William Dalleywater; Alexander V. Predeus; Batuhan Cakir; Pavel Mazin; Jayakumar Vadakekolathu; Sergio Rutella; Marian L. Meakin; Alison A. Ritchie; Shamir Montazid; Sara Cuevas Ocaña; Nadine Holmes; Victoria Wright; Fei Sang; Silvia Santoni; Adam Bills; Declan Sculthorpe; Rasa Elmentaite; Sarah A. Teichmann; Shazia Irshad; Ian Tomlinson; Andrew Silver; Ricky D. Wildman; Nicholas R. F. Hannan; Felicity R. A. J. Rose; Mohammad Ilyas

DOI: 10.1186/s13287-026-05006-4Status: Verified Translated Edition

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Key Findings in This Report

• Developed a rapid 8-day serum-free co-differentiation platform from hiPSCs generating epithelial, mesenchymal, and endothelial intestinal lineages. • Derived cells exhibit intestinal identity with early colonic polarization, confirmed by bulk and single-cell RNA sequencing. • Colon-like intestinal patches (CL-IPs) formed organized tissue structures with human vasculature that anastomosed with host murine vessels after transplantation. • No residual pluripotency detected, supporting safety for potential regenerative therapy applications.