Evaluation of secretomes derived from human dermal and adipose tissue mesenchymal stem/stromal cells for skin wound healing: not as effective as cells

• Secretomes from dermal and adipose MSCs show overlapping protein composition but differential expression of wound healing and angiogenesis-related proteins. • In a murine skin wound model, secretomes were less effective than their parental cells in accelerating wound closure and tissue remodeling. • Secretome-treated wounds exhibited intermediate outcomes between cell-treated and control groups, indicating partial therapeutic benefit. • Findings underscore the need for protocol optimization to enhance secretome efficacy for clinical translation in regenerative medicine.