Efficient production of recombinant mAbs mediated by a MAR-enhanced transposon vector combined with blasticidin selection in CHO cells

• The MAR-PB transposon system combined with blasticidin selection significantly increases monoclonal antibody titers (3.95- to 5.61-fold) and specific productivity (4.28- to 6.07-fold) compared to random integration. • This approach enhances the proportion of high-expressing clones by more than 10-fold and ensures stable recombinant antibody expression for over 60 generations. • Transcriptome analysis indicates that the system modulates genes involved in DNA binding, transcriptional regulation, and protein binding, contributing to improved expression. • The MAR-PB system offers a rapid and efficient method for generating stable, high-yielding CHO cell clones for industrial recombinant antibody production.