Efficacy of Multi-Layered Human iPS Cell-Derived Cardiovascular Cell Sheets in a Pacing-Induced Canine Dilated Cardiomyopathy Model

• A novel Step-Down Pacing Heart Failure (SDPHF) canine model maintains stable heart failure for at least 4 weeks without mortality, enabling reliable evaluation of therapies. • IHJ-301, a multi-layered iPSC-derived cardiovascular cell sheet product, significantly improved left ventricular ejection fraction, fractional shortening, stroke volume, and cardiac output in the DCM model. • The SDPHF model addresses limitations of previous pacing models by preventing spontaneous recovery and premature death, providing a robust platform for preclinical testing. • These results provide strong preclinical evidence supporting the potential of IHJ-301 as a therapeutic option for dilated cardiomyopathy.