• DGKγ is predominantly expressed in developing neural tubes and co-localizes with neural stem cell markers.
• Knockdown of DGKγ or inhibition with R59949 reduces NSC proliferation and migration, while PMA (DAG analog) increases proliferation but impairs migration.
• DGKγ regulates NSC migration via the DAG/PKCδ pathway, as evidenced by increased DAG content and p-PKCδ/PKCδ ratio upon DGKγ inhibition.
• These findings highlight DGKγ as a potential therapeutic target for neural tube defects and neurodevelopmental disorders.