Official PDF Translation•Acta Biochimica et Biophysica Sinica

Development of a Colloidal Gold Immunochromatographic Strip Based on GAPDH for Pentatrichomonas hominis in Dogs

Authors: Qian Zhai; Xuejiao Chen; Xichen Zhang; Jianhua Li; Pengtao Gong; Xiaocen Wang; Xin Li; Xu Zhang; Nan Zhang

DOI: 10.3724/abbs.2026043Status: Verified Translated Edition

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Key Findings in This Report

• Identified GAPDH as a novel immunogenic and species-specific antigen for P. hominis detection using LC-MS/MS. • Developed a colloidal gold immunochromatographic strip based on recombinant GAPDH for rapid on-site detection of P. hominis in dogs. • The strip offers high sensitivity and specificity, overcoming limitations of microscopy and PCR. • Provides a practical tool for veterinary practice and public health surveillance of P. hominis infections.