• Blastocyst complementation enables in vivo organ generation by introducing pluripotent stem cells into organogenesis-disabled embryos, leveraging natural developmental cues.
• Interspecies application holds promise for producing human organs in livestock, potentially alleviating the global organ shortage crisis.
• The review highlights key challenges including ethical considerations, chimerism efficiency, and interspecies barriers that must be overcome for clinical translation.
• Current alternatives like xenotransplantation and tissue engineering face limitations, underscoring the need for innovative approaches like blastocyst complementation.