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Open AccessDOI: 10.12307/2026.21394Original Research

Biomechanical analysis during non-anticipated stop-jump cutting before and after exercise fatigue in functional ankle instability and healthy populations

Fu Guangliang¹,Bao Chunyu¹,Meng Qinghua¹,Wang Baochen¹,Cao Jiaxing¹,Sun Jiawei¹

Tianjin University of Sport

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Biomechanical analysis during non-anticipated stop-jump cutting before and after exercise fatigue in functional ankle instability and healthy populations
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Published In
Chinese Journal of Tissue Engineering Research
Published:January 15, 2026Edition:Vol 1902, Issue 30 • pp. 100-112Citation:Fu Guangliang et al. (2026), Chinese Journal of Tissue Engineering Research
Impact FactorPremier Chinese Biomedical Journal indexed in SinoBioData: Chinese Journal of Tissue Engineering Research (中国组织工程研究).
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Key Takeaways & Executive Findings

  • • Exercise fatigue increases ankle inversion and alters lower limb joint kinematics during stop-jump cutting, particularly in individuals with functional ankle instability. • Fatigue reduces hip, knee, and ankle joint stiffness, impairing dynamic stability and shock absorption, especially in the functional ankle instability group. • Statistical parametric mapping revealed time-specific changes in ankle angle and ground reaction forces, highlighting critical phases of increased injury risk. • Functional ankle instability individuals exhibit altered movement patterns under fatigue, potentially explaining their higher risk of recurrent ankle sprains.
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Abstract

BACKGROUND: Systemic fatigue increases injury risk in individuals with functional ankle instability, while stop-jump cutting is a high risk for ankle injuries. The biomechanical mechanisms underlying non-anticipated stop-jump cutting during systemic exercise fatigue in this population remain unclear. OBJECTIVE: To quantify differences in kinematic and kinetic characteristics during non-anticipated stop-jump cutting before and after exercise fatigue between individuals with functional ankle instability and healthy controls, revealing the impact of exercise fatigue on stop-jump cutting in individuals with functional ankle instability. METHODS: Fifteen male participants with unilateral functional ankle instability and 15 healthy male controls were recruited. Kinematic (peak angles of ankle dorsiflexion, plantarflexion, inversion, knee flexion, knee varus, knee valgus, hip flexion, and hip abduction) and kinetic (joint stiffness of hip, knee, and ankle) parameters were collected during non-anticipated stop-jump cutting before and after exercise fatigue. Two-way repeated measures ANOVA was used to analyze peak joint angles and joint stiffness. Statistical parametric mapping (SPM) was further used to analyze the effects of fatigue on time-series data of ankle angle and ground reaction forces. RESULTS AND CONCLUSION: Kinematic results: Two-way ANOVA revealed significant main effects of fatigue and group-by-fatigue interactions for peak ankle inversion, knee flexion, knee valgus, and hip flexion angles (P < 0.05). Compared with pre-fatigue, peak ankle inversion increased in the functional ankle instability group after fatigue (P < 0.05), peak knee flexion increased in both groups (P < 0.05), and peak hip flexion increased in the healthy control group (P < 0.05). After fatigue, the functional ankle instability group showed smaller peak ankle inversion and hip flexion angles but larger peak knee valgus and knee flexion angles than the healthy control group (P < 0.05). SPM analysis revealed that ankle inversion/eversion angle was greater during 4%-18% of the cutting movement after fatigue in the functional ankle instability group (P < 0.05). Kinetic results: Two-way ANOVA revealed significant main effects of fatigue and group-by-fatigue interactions for hip, knee, and ankle joint stiffness (P < 0.05). Compared with pre-fatigue, hip and ankle stiffness decreased in the healthy control group (P < 0.05), while knee and ankle stiffness decreased in the functional ankle instability group (P < 0.05). SPM analysis showed that vertical ground reaction force was greater during 5%-16% of the cutting movement, and mediolateral ground reaction force was greater during 35%-49% of the movement after fatigue in the functional ankle instability group (P < 0.05). CONCLUSION: Exercise fatigue alters kinematic and kinetic characteristics during non-anticipated stop-jump cutting in individuals with functional ankle instability, particularly affecting knee and ankle stability and shock absorption. Fatigue reduces joint stiffness and control, increasing injury risk, especially during the initial and transitional phases of the cutting movement.

1. Introduction

Ankle injuries are extremely common in sports, and the risk of re-injury after an initial ankle sprain is significantly elevated. The mechanical mechanisms underlying this condition remain unclear, making it a key research topic in sports medicine. Statistics show that the recurrence rate of ankle sprains can be as high as 80%, with approximately 30% of patients developing functional ankle instability (FAI). The concept of FAI was first proposed by Freeman in 1965, describing a subjective feeling of instability despite structural healing, reflecting incomplete neuromuscular recovery.

FAI is associated with various sensorimotor deficits, including decreased postural stability, impaired proprioception, reduced plantar tactile sensitivity, and weakened ankle musculature. Due to repeated sprains, mechanoreceptors and afferent nerves are damaged, leading to reduced afferent information, which impairs the central nervous system's integration of posture and movement, thereby diminishing neuromuscular responses to perturbations. This creates a vicious cycle of 'sensory impairment - control deficit - re-injury - increased instability,' which has long been a focus in sports medicine and biomechanics.

Stop-jump cutting is a fundamental athletic maneuver widely performed in sports like basketball and soccer. During this action, ground reaction forces can reach up to three times body weight, posing significant challenges to dynamic stability and motor control of the lower limb joints. Studies have shown that individuals with FAI exhibit abnormally increased ankle inversion angles and altered ground reaction forces during cutting, which may be a key mechanism for recurrent ankle sprains.

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Cite This Research Paper
Fu Guangliang, Bao Chunyu, Meng Qinghua, Wang Baochen, Cao Jiaxing, Sun Jiawei (2026). Biomechanical analysis during non-anticipated stop-jump cutting before and after exercise fatigue in functional ankle instability and healthy populations. Chinese Journal of Tissue Engineering Research. https://doi.org/10.12307/2026.21394
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Frequently Asked Questions

What is functional ankle instability (FAI)?

Functional ankle instability is a condition characterized by a subjective feeling of ankle instability and recurrent sprains despite structural healing. It is associated with neuromuscular and sensorimotor deficits, such as impaired proprioception and postural control.

How does exercise fatigue affect biomechanics during stop-jump cutting in FAI individuals?

Exercise fatigue increases ankle inversion angles and reduces joint stiffness in the lower limb, particularly at the knee and ankle, during stop-jump cutting. This impairs dynamic stability and shock absorption, increasing the risk of injury, especially during the initial and transitional phases of the movement.

What is statistical parametric mapping (SPM) and how is it used in this study?

Statistical parametric mapping is a method for analyzing continuous time-series data, such as joint angles and ground reaction forces, to identify specific time periods where significant differences occur. In this study, SPM was used to compare biomechanical data before and after fatigue, revealing time-specific changes in ankle angle and ground reaction forces.

What are the clinical implications of this research?

The findings highlight that fatigue exacerbates biomechanical risk factors in individuals with FAI, such as increased ankle inversion and reduced joint stiffness. This suggests that injury prevention programs should incorporate fatigue management and neuromuscular training to improve joint stability and control during high-risk movements like cutting.

What are the limitations of this study?

The study only included male participants, and the fatigue protocol may not fully replicate real-world sports fatigue. Future research should include female participants and use more sport-specific fatigue protocols, as well as incorporate electromyography to explore neuromuscular control mechanisms.

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