• Established a visualized 3D culture model of mouse fetal ovaries using GFP-BVSC reporter, enabling non-invasive monitoring of ovarian development.
• Quercetin supplementation reduces ROS and protects mitochondria, improving tissue area and follicle count in cultured ovaries.
• The platform effectively models fetal reproductive aberrations caused by gestational diabetes mellitus (GDM).
• Provides a simplified, efficient tool for drug screening and potential clinical treatment of ovarian hypofunction.