• GenoCare 1600 is a novel single-molecule desktop sequencer using amplification-free library preparation and two-color sequencing-by-synthesis, offering a user-friendly alternative for clinical use.
• The platform achieves consensus accuracy exceeding 99.99% on E. coli standard samples, with sequencing completed within 15 hours and an average read length of 53 bp.
• GenoCare enables accurate microbial quantitation, sensitive SARS-CoV-2 detection, and reliable variant identification in COVID-19 throat swab samples, validated by Sanger sequencing.
• The technology addresses limitations of existing single-molecule platforms (e.g., SMRT, nanopore) by providing higher throughput (over 300 million reads per cell) and reduced cost and time, making it suitable for clinical applications like NIPT and pathogen detection.