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Protected by Google reCAPTCHA v3.•Privacy•Terms Key Findings in This Report
• Optimized enzymatic digestion parameters (0.4 PZ U/mL Collagenase NB6, 3 h) significantly increase P0 WJ-MSC yield.
• Enzymatic digestion outperforms the explant method in initial outgrowth, and passages 2-5 show superior viability and proliferation.
• Successful scale-up from laboratory flasks to pilot-scale cell factories demonstrates GMP-compliant manufacturing feasibility.
• Stability studies reveal that multiple freeze-thaw cycles and storage at 20–27°C reduce drug product viability, emphasizing careful handling.