Zizhu ointment enhances wound healing in diabetic ulcer mice via angiogenesis regulation

• Zizhu ointment significantly accelerates wound healing in diabetic ulcer model mice, with faster healing observed at days 3, 7, 11, and 14 compared to model controls. • Treatment with Zizhu ointment enhances angiogenesis, as evidenced by increased microvessel density and elevated expression of pro-angiogenic factors VEGFA and ANGPT2. • Zizhu ointment downregulates the expression of angiogenesis inhibitors SPRED1 and PIK3R2, suggesting a mechanism involving the modulation of angiogenic signaling pathways. • The study provides evidence that topical application of Zizhu ointment may be an effective therapeutic strategy for diabetic ulcers by promoting wound angiogenesis.