UBE2C promotes myoblast differentiation and skeletal muscle regeneration through the Akt signaling pathway

• UBE2C expression is upregulated during embryonic skeletal muscle development, suggesting a role in myogenesis. • Knockdown of UBE2C inhibits C2C12 myoblast differentiation and reduces myogenic markers MyoG and MyHC, while overexpression enhances differentiation. • In vivo knockdown of UBE2C in the tibialis anterior muscle severely impairs muscle regeneration. • UBE2C regulates myogenesis via the Akt signaling pathway by modulating Akt phosphorylation and stability.