Total flavonoids of litchi seed attenuates cellular senescence by inhibiting the production of SASP through p65 suppression and ameliorates pulmonary fibrosis

• TFL delays replicative and stress-induced cellular senescence and alleviates SASP. • TFL reduces bleomycin-induced DNA damage, pulmonary senescence, and fibrosis in mice. • Mechanistically, TFL suppresses p65 expression, inhibiting IL-1α/IL-1β and SASP. • TFL reverses bleomycin-induced gut microbiome alterations, suggesting a role in gut-lung axis.