• Cofilin1 is highly expressed in bladder cancer and metastatic lymph nodes, correlating with tumor grade and poor patient survival.
• Cofilin1 promotes bladder cancer cell proliferation and migration by binding to cortactin, reducing F-actin, and inducing invadopodia formation.
• TCF7L2 transcriptionally activates Cofilin1 and suppresses miR-206 maturation, forming a TCF7L2/miR-206/Cofilin1 axis that drives metastasis.
• The TCF7L2/miR-206/Cofilin1 pathway offers novel therapeutic targets for T1G3 bladder cancer, potentially improving treatment strategies and prognosis.