• This special issue compiles 12 expert reviews covering RNA biogenesis, functions, and technologies, highlighting the diversity and complexity of the RNA landscape.
• Reviews discuss alternative splicing regulation by RNA structures and its impact on disease, as well as crosstalk between splicing and 3'-end processing.
• Frontier topics include RNA modifications (e.g., m6A) and editing, with structural insights into writers and links to disease.
• Technological advances in RNA proximity labeling, fluorogenic aptamers, and long-read sequencing are presented as powerful tools for studying RNA dynamics and regulation.