RTCB deficiency triggers colitis in mice by influencing the NF-κB and Wnt/β-catenin signaling pathways

• RTCB deficiency in mouse colon disrupts intestinal barrier integrity and induces inflammatory cell infiltration, leading to colitis. • RTCB knockout activates the NF-κB pathway, evidenced by increased TNF-α, IL-8, and p-p65, and decreased IKKβ and IκBα. • RTCB deficiency suppresses the Wnt/β-catenin pathway, with reduced Wnt3a, β-catenin, and LGR5, and increased GSK3β. • The study provides novel insights into RTCB's role in colonic homeostasis and suggests potential therapeutic targets for colitis.