• • Compound X at 20 mg/kg reduced myocardial infarct size by 58.2% (from 45.2% to 18.9%, p<0.05) in a rat I/R model, indicating potent cardioprotection.
• • Nrf2 nuclear translocation increased 2.3-fold and HO-1 expression upregulated 3.1-fold at 20 mg/kg, correlating with reduced oxidative stress.
• • MDA levels decreased from 8.2 to 5.1 nmol/mg protein (38% reduction) and SOD activity increased from 12.3 to 21.6 U/mg protein (75% increase) at 20 mg/kg, demonstrating robust antioxidant effects.
• • Apoptotic index (TUNEL-positive cells) was reduced by 62% at 20 mg/kg, with a 45% decrease in Bax/Bcl-2 ratio, indicating significant anti-apoptotic activity.