OSBPL2-Mediated Lipid Metabolism Alteration Governs Lung Cancer Stem Cells Properties

• OSBPL2 is downregulated in lung cancer tissues and its low expression correlates with poor patient survival, suggesting a tumor suppressor role. • OSBPL2 reduces cholesterol content and inhibits lipid droplet accumulation in lung cancer cells, altering lipid metabolism. • OSBPL2 suppresses lung cancer stemness properties, including tumor sphere formation, stemness marker expression, and in vivo tumorigenesis and metastasis. • OSBPL2 expression negatively correlates with malignant features such as tumor stage progression and lymph node metastasis, highlighting its potential as a therapeutic target.