Metabolic Engineering of Methanotrophs for Production of Methionine and Its Derivatives

• Engineered methanotroph Methylococcus capsulatus (Bath) to produce methionine at 2.5 g/L from methane. • Introduced heterologous methionine biosynthetic pathway and optimized metabolic flux to achieve high yield. • Demonstrated production of methionine derivatives (sulfoxide and sulfone) as feed additives. • Provides a sustainable approach for converting greenhouse gas methane into valuable amino acids.