Long noncoding RNA HNF1A-AS1 promotes ovarian cancer growth and M2 macrophage polarization by counteracting miR-214-mediated suppression of semaphorin 4D signaling

• HNF1A-AS1 is upregulated in epithelial ovarian cancer (EOC) cells and acts as a competing endogenous RNA (ceRNA) by sponging miR-214, thereby relieving miR-214-mediated suppression of SEMA4D signaling. • Overexpression of HNF1A-AS1 promotes EOC cell proliferation, migration, invasion, and tumor growth in xenograft models, while knockdown suppresses these malignant phenotypes. • HNF1A-AS1 drives M2 macrophage polarization in the tumor microenvironment, an effect that is reversed by miR-214 overexpression or SEMA4D silencing, highlighting a novel immunomodulatory mechanism. • The HNF1A-AS1/miR-214/SEMA4D axis represents a promising therapeutic target for EOC, offering potential for combined anti-tumor and immunomodulatory strategies.