Efficient production of recombinant mAbs mediated by a MAR-enhanced transposon vector combined with blasticidin selection in CHO cells

• The MAR-PB transposon system combined with blasticidin selection significantly enhances monoclonal antibody titers (up to 5.61-fold) and specific productivity (up to 6.07-fold) in CHO cells compared to random integration. • This approach increases the proportion of high-expressing clones by over 10-fold and ensures stable recombinant antibody expression for more than 60 generations, addressing long-term production stability. • Transcriptome analysis reveals that the system modulates genes involved in DNA binding, transcriptional regulation, and protein binding, providing mechanistic insights into enhanced expression. • The MAR-PB system offers a rapid and efficient method for generating stable, high-yielding CHO cell clones, representing a valuable tool for industrial recombinant antibody production.