• hiPSC-derived extracellular vesicles (EVs) attenuate hyperoxia-induced airspace enlargement and improve parenchymal histology in a fetal murine lung explant model.
• Differentiated hiPSC-derived EVs (diPSC-EVs) upregulate VEGFa and antioxidant genes, suggesting pro-angiogenic and cytoprotective potential.
• EV proteomic profiling identifies pathways related to alveolarization, angiogenesis, and anti-inflammatory/regenerative processes.
• This proof-of-concept study supports a cell-free EV-based approach for preventing or treating Bronchopulmonary Dysplasia (BPD).