Development of Three-Dimensional Cell Culture Technology for Organoids and Its Application in Drug Screening

• Organoids recapitulate key aspects of human organ physiology, offering superior models for drug screening compared to 2D cultures. • Advanced 3D culture techniques, including bioprinting and microfluidics, enhance organoid complexity and maturation. • Integration of organoids with CRISPR and high-throughput screening accelerates drug discovery and personalized medicine. • Overcoming challenges like vascularization and reproducibility is crucial for translating organoid technology to clinical applications.