Development of a colloidal gold immunochromatographic strip based on GAPDH for Pentatrichomonas hominis in dogs

• Identified GAPDH as a novel immunogenic and species-specific antigen for Pentatrichomonas hominis using LC-MS/MS proteomics. • Developed a colloidal gold immunochromatographic strip based on recombinant GAPDH for rapid, on-site detection of P. hominis in dogs. • The strip offers a practical alternative to PCR and microscopy, with potential for field use and point-of-care diagnostics. • This is the first report of an immunoassay for P. hominis detection, addressing the lack of specific antigens and facilitating epidemiological studies.