Coupling of alternative splicing and alternative polyadenylation

• Crosstalk between splicing and 3′-end processing is bidirectional, with each process influencing the other through molecular mechanisms. • 3′-end processing factors and polyA tail promote splicing of the last intron, and can also affect internal and terminal exon splicing. • Splicing within the 3′ UTR contributes to 3′ UTR diversity, and splicing factors can influence 3′-end processing. • Long-read sequencing technologies are crucial for understanding the coordination of AS-APA events and may aid in disease diagnosis and treatment.