• Successfully constructed a vascular endothelial growth factor receptor 2 (VEGFR2) gene knockdown rat model using adeno-associated virus (AAV) technology.
• In situ injection of VEGFR2 knockdown AAV achieved a 60% knockdown efficiency in rat muscle tissue.
• VEGFR2 knockdown in muscle tissue led to a significant reduction in muscle fiber area and microvessel number.
• The study provides a valuable experimental basis for further research on VEGFR2's role in cervical spondylosis and potential therapeutic strategies.