Constructing an in vitro model of ulcerative colitis in mice based on organoid technology

• A mouse colon organoid model of ulcerative colitis was successfully established using lipopolysaccharide induction. • Lipopolysaccharide at concentrations of 225-275 μg/mL significantly increased pro-inflammatory cytokines (IL-6, TNF-α) and decreased tight junction protein expression. • The organoid model recapitulates key features of ulcerative colitis, including epithelial barrier disruption and inflammatory response. • This model provides a valuable platform for drug screening and mechanistic studies of ulcerative colitis.