Combined Proteomics and Metabolomics Analysis of Pathological Mechanisms in Mouse Models of Coronary Heart Disease

• Integrated proteomics and metabolomics identified 420 differentially expressed proteins and 155 differential metabolites in a mouse model of coronary heart disease, revealing 26 protein-metabolite correlations. • The study provides molecular evidence linking coronary heart disease to energy metabolism disorders, inflammatory-coagulation cascade activation, and ion homeostasis imbalance, bridging traditional Chinese medicine 'blood stasis' syndrome with modern molecular mechanisms. • Potential diagnostic markers and therapeutic targets were identified, including serum amyloid A protein and neutrophil elastase as inflammatory markers, and cytochalasin B and protein kinase D agonists as candidates for vascular remodeling and myocardial repair. • The findings underscore the value of multi-omics approaches in uncovering complex disease mechanisms and offer new avenues for syndrome differentiation and drug development in coronary heart disease.