• Imaging mass cytometry reveals a complex multicellular architecture in psoriatic lesions, with diverse immune subsets, endothelial cells, keratinocytes, and nerve fibers.
• Spatial neighborhood analysis identifies enrichment of immune cells and nerve fibers around keratinocytes, suggesting potential cellular crosstalk.
• Positive interactions between cutaneous nerve fibers and CD8+ T cells, CD68+ macrophages, and blood vessels highlight neuroimmune involvement in psoriasis.
• Epigenetic marker H3K27me3 is spatially mapped in immune cells adjacent to keratinocytes, linking epigenetic regulation to the psoriatic microenvironment.