Stem Cell Research & Therapy•2024•DOI: 10.1186/s13287-024-03745-w
Background Biomaterials used in bone tissue engineering must fulfill the requirements of osteoconduction, osteoinduction, and osseointegration. However, biomaterials with good osteoconductive properties face several challenges, including inadequate vascularization, limited osteoinduction and barrier ability, as well as the potential to trigger immune and inflammatory responses. Therefore, there is an urgent need to develop guided bone regeneration membranes as a crucial component of tissue engineering strategies for repairing bone defects. Methods The mZIF-8/PLA membrane was prepared using electrospinning technology and simulated body fluid external mineralization method. Its ability to induce biomimetic mineralization was evaluated through TEM, EDS, XRD, FT-IR, zeta potential, and wettability techniques. The biocompatibility, osteoinduction properties, and osteo-immunomodulatory effects of the mZIF-8/PLA membrane were comprehensively evaluated by examining cell behaviors of surface-seeded BMSCs and macrophages, as well as the regulation of cellular genes and protein levels using PCR and WB. In vivo, the mZIF-8/PLA membrane's potential to promote bone regeneration and angiogenesis was assessed through Micro-CT and immunohistochemical staining. Results The mineralized deposition enhances hydrophilicity and cell compatibility of mZIF-8/PLA membrane. mZIF-8/PLA membrane promotes up-regulation of osteogenesis and angiogenesis related factors in BMSCs. Moreover, it induces the polarization of macrophages towards the M2 phenotype and modulates the local immune microenvironment. After 4-weeks of implantation, the mZIF-8/PLA membrane successfully bridges critical bone defects and almost completely repairs the defect area after 12-weeks, while significantly improving the strength and vascularization of new bone. Conclusions The mZIF-8/PLA membrane with dual osteoconductive and immunomodulatory abilities could pave new research paths for bone tissue engineering.
Chinese Traditional and Herbal Drugs•2026•DOI: 10.7501/j.issn.0253-2670.2026.16.20261630
High altitude disease (HAD) arises from rapid ascent to elevations exceeding 2,500 m, where hypobaric hypoxia, cold, dryness, and intense ultraviolet radiation converge to produce a complex syndrome frequently manifesting as headache, dizziness, emesis, fatigue, and dyspnea; severe cases progress to high altitude pulmonary edema (HAPE) or high altitude cerebral edema. Epidemiological data indicate a combined HAD incidence of 37% in China, with acute high altitude disease (AHAD) alone reaching 40%, imposing a substantial public health burden. Conventional management relies on staged ascent, oxygen supplementation, and symptomatic pharmacotherapy, yet adverse effects and restricted applicability limit these interventions. Rhodiolae Crenulatae Radix et Rhizoma, the dried root and rhizome of Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba, has been used since the Eastern Han Dynasty and is classified in the Tibetan medical canon as one of the 'Three Auspicious Treasures.' Its phytochemical profile encompasses phenylethanoid glycosides, flavonoids, phenylpropanoids, volatile oils, and organic acids. Modern pharmacology confirms anti-inflammatory, anti-oxidative stress, anti-fatigue, programmed cell death-regulatory, glucose-lipid metabolic, and gut microbiota-modulatory activities. This review synthesizes recent mechanistic and clinical evidence, providing a reference for clinical translation of Rhodiola crenulata in HAD management.
Chinese Journal of Tissue Engineering Research•2026•DOI: 10.12307/2026.21602
BACKGROUND: 4D printing enables dynamic control of structure and function, allowing constructs to more closely mimic complex physiological environments and driving the development of tissue engineering and regenerative medicine towards intelligence and personalization. OBJECTIVE: To review the role and application advances of 4D printing in tissue engineering and regenerative medicine. METHODS: Relevant literature published between 1994 and 2025 was retrieved from CNKI, WanFang, PubMed, and Web of Science. Chinese and English terms were “4D printing, regenerative medicine, tissue engineering, wound healing, biological ink, intelligent materials.” A total of 115 articles were systematically reviewed and analyzed. RESULTS AND CONCLUSION: 4D bioprinting represents a key advance in tissue engineering and regenerative medicine, integrating diverse fabrication methods and biomaterials to create structures that dynamically respond to environmental cues. 4D bioprinting enhances the biomimicry of natural tissues, enables customized responsiveness, and improves integration with biological systems. 4D bioprinting facilitates the development of tissues and organs, as well as intelligent implants and advanced drug delivery systems, ultimately for tissue repair. 4D bioprinting can seamlessly adapt to the physiological complexity of the human body, apply to personalized medicine, and significantly improve therapeutic outcomes in changing environments.