Genomics, Proteomics & Bioinformatics•2024•DOI: 10.1093/gpbjnl/qzae006
DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years. In this study, we developed a single-molecule desktop sequencer, GenoCare 1600 (GenoCare), which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry, making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use. Using the GenoCare platform, we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%. We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019 (COVID-19) samples from throat swabs. Our findings indicate that the GenoCare platform allows for microbial quantitation, sensitive identification of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, and accurate detection of virus mutations, as confirmed by Sanger sequencing, demonstrating its remarkable potential in clinical application.
Chinese Traditional and Herbal Drugs•2026•DOI: 10.7501/j.issn.0253-2670.2026.16.20261618
This study systematically interrogated the China National Intellectual Property Administration patent database to identify candidate core herb combinations and therapeutic targets for ischemic stroke (IS), integrating cluster analysis, network pharmacology, molecular docking, molecular dynamics simulations, single-cell RNA sequencing, spatial transcriptomics, and CellOracle-based in silico knockdown. A core herb combination of Polygalae Radix, Acori Tatarinowii Rhizoma, Rhei Radix et Rhizoma, and Curcumae Radix was identified, yielding nine representative active components (1-hydroxyacoronene, aristolone, calamendiol, β-asarone, α-asarone, kaempferol, thymol, eugenol, caffeic acid) and six candidate targets (TNF, IL-6, AKT1, EGFR, MAPK1, PTGS2). Molecular docking and dynamics simulations indicated binding tendencies, with kaempferol-PTGS2 and kaempferol-EGFR complexes showing stability under simulated conditions. Single-cell and spatial transcriptomics revealed spatial concordance between high candidate target expression regions and macrophage/astrocyte distribution. CellOracle predicted that Jun/Fos and STAT3 transcription factors may regulate post-ischemic inflammatory responses and cell state transitions. The study constructs a mechanistic evidence chain linking core herbs, active components, candidate targets, and spatiotemporal validation, offering a modern biological interpretation of the 'Tongfu Xingshen' therapeutic principle. However, findings remain computational and require experimental validation; limitations include single-timepoint single-cell data (24 h post-MCAO), reliance on public databases, and lack of in vivo/in vitro efficacy experiments.